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KMID : 1144820180240030253
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대한의생명과학회지
2018 Volume.24 No. 3 p.253 ~ p.262
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Inhibitory Effects of PD98059, SB203580, and SP600125 on α- and δ-granule Release and Intracellular Ca2+ Levels in Human Platelets
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Kwon Hyuk-Woo
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Abstract
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Platelets are activated at sites of vascular injury via several molecules, such as adenosine diphosphate, collagen and thrombin. Full platelet aggregation is absolutely essential for normal hemostasis. Moreover, this physiological event can trigger circulatory disorders, such as thrombosis, atherosclerosis, and cardiovascular disease. Therefore, platelet function inhibition is a promising approach in preventing platelet-mediated circulatory disease. Many studies reported the involvement of mitogen-activated protein kinases (MAPKs) signaling pathways in platelet functions. However, these studies were limited. Thus, we examined MAPK signaling pathways in human platelets using specific MAPK inhibitors, such as PD98059, SB203580, and SP600125. We observed that these inhibitors were involved in calcium mobilization and influx in human platelets. They also suppressed thrombin-induced α- and δ-granule release. These results suggest that PD98059, SB203580, and SP600125 exhibit Ca2+ antagonistic effects.
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KEYWORD
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MAPK signaling pathways, Calcium mobilization, Calcium influx, Granule release
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